![]() The verdict? There is no outstanding evidence anywhere that says that you should or shouldn't wear a bra to sleep, so the best course of action when deciding whether to wear it or not is to review all the facts. Removing one's bra is the best, most relaxing part of the day for some of us, but why is sleeping in a bra bad for you exactly? While some experts would encourage bra-goers to shed their bras off at night, others would suggest that you not remove your bra while you sleep. And by "fun," I mean go home, take off our bras, put our sweats on, and watch Pretty Little Liars. With additional reporting by Cathy Bishop.At the end of a long day of work, the only thing us girls want to do is have fun. Need help finding your perfect sleep bra? Contact our expert team of Fit Stylists for help. If you feel more comfortable with extra support at night, make sure that you choose a comfortable, seamless, and tagless wireless bra for maximum comfort. Stretchy cotton or moisture wicking fabrics.Pull on style with no fastenings (like a lightweight sports bra).Here are some key features to look out for when choosing an ultra-cozy sleep bra: For nighttime support that won’t interrupt your sleep, choose a comfortable wireless style that’s soft and moves with you. And if you want additional support while sleeping, choose a comfortable bra that won’t interrupt your slumber.Īlthough most typically prefer sleeping without a bra, those with larger breasts might find lightweight structure beneficial, especially if they toss and turn while sleeping. Moral of the story: let ‘em loose! Your breasts will thank you. “Overall wearing a bra at night doesn’t help with preventing breast tissue from sagging and it can hinder in many ways, including reducing your sleep quality,” Dr. To make sure your bra isn’t too tight, you should be able to slip two fingers between the band and your ribcage. That’s why it’s important to be sure you’re not wearing a bra that’s restrictive. ![]() Not only is it unnecessary, but sleeping in a tight bra can also cause the following issues, according to Dr. “At night, our breast tissue doesn’t have the gravitational pull as we are lying down, so we don’t need to wear a bra for prevention reasons,” she said. Mysore explained that the connective tissue in breast tissue naturally loosens over time (aka sagging) which can be combatted by wearing a bra during the day. To get a professional opinion, we spoke with Navya Mysore, MD, Medical Director at One Medical Group. Is sleeping in your bra bad for your health? There’s a lot of ground to cover here, so keep reading to learn more. This one can be true, depending on your bra. Sleeping in a bra is bad for your health.This is completely false! There is absolutely no connection between bras and breast cancer. Wearing a bra when sleeping causes breast cancer.This is a complete myth and there is no science to back up the claim that bras impact breast size. There are several rumors claiming that wearing a bra restricts blood circulation and prevents breast growth. Sleeping in your bra stops breast tissue growth.Wearing a supportive bra in the right size can help you feel comfortable and prevent back and shoulder pain, but sleeping in one will neither cause nor prevent sagging. Your breasts can sag for a variety of reasons such as pregnancy, weight fluctuations, aging, and more. These are both myths! Just as your body changes throughout your life, so do your breasts. Wearing a bra at night causes or prevents sagging.(Only Marilyn knows for sure.) Let’s debunk some common bra-related myths! (It depends.) And Marilyn Monroe was reported to have slept in a bra every night. (It doesn’t.) Others say it’s bad for your health. When it comes to sleeping with a bra on, rumors abound. It’s time to settle this debate once and for all: to wear or not to wear a bra to bed. We’ve worked with thousands of women to find their perfect fit, so we’re sharing some of our best advice here. Our team of Fit Stylists is here to help you find bras that feel like they were made for you.
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Uses the current working directory as the build tree, and as the Run CMake with one of the following command signatures to specify the source and build When using one of the IDE Build Tool Generators, no particular environment is When using one of the Command-Line Build Tool Generators CMake expects that theĮnvironment needed by the compiler toolchain is already configured in the shell. Generator, or simply accept the default CMake chooses for the current platform. Optionally use the -G option below to specify a Manual for documentation of all generators. This chooses the kind of buildsystem to generate. ![]() Placed in the same directory as the source tree is also supported, but discouraged. An in-source build in which the build tree is To maintain a pristine source tree, perform an out-of-source build by using a Such as buildsystem configuration options. CMake will write a CMakeCache.txtįile to identify the directory as a build tree and store persistent information The top-level directory in which buildsystem files and build output artifacts (e.g.Įxecutables and libraries) are to be stored. These files specifyīuild targets and their dependencies as described in the cmake-buildsystem(7) Manual, starting with a top-level file named CMakeLists.txt. Project specifies its buildsystem using files as described in the cmake-language(7) The top-level directory containing source files provided by the project. To generate a buildsystem with CMake, the following must be selected: From these files CMake generates a preferred buildsystem locally for each user In order to avoid maintaining multiple such buildsystems,Ī project may specify its buildsystem abstractly using files written in the CMake language. ![]() Makefile for use with a command-line make tool or a project file for an Integratedĭevelopment Environment (IDE). For command-line interfaces to the CMake testing and packaging facilities, seeįor more information on CMake at large, see also the links at the end of this manual.Ī buildsystem describes how to build a project’s executables and libraries from its sourceĬode using a build tool to automate the process. The other actions are meant for use by software developers writing scripts in the CMake language to support their builds.įor graphical user interfaces that may be used in place of cmake, see ccmake andĬmake-gui. Optionally useĬmake to Build a Project, Install a Project or just run the corresponding build tool (e.g. To build a software project with CMake, Generate a Project Buildsystem. ![]() The above Synopsis lists various actions the tool can perform as The cmake executable is the command-line interface of the cross-platform buildsystem ![]() ![]() In a semi-dry protein transfer, the transfer sandwich is placed horizontally between two plate electrodes. ![]() Semi-dry electroblotting (Semi-dry transfer) With increasing time, however, there is a risk of over-transfer (stripping, blow through) of the proteins through the membrane, especially for lower molecular weight (<30 kDa) proteins when using membranes with a larger pore size (0.45 µm). The transfer efficiency improves with increased transfer time and is better, in general, for lower molecular weight proteins than higher molecular weight proteins. Transfer efficiencies of 80–100% are achievable for proteins between 14–116 kDa. A high field option exists for transferring a single gel, which may bring transfer time down to as little as 30 minutes, but it requires the use of high voltage (up to 200 V) or high current (up to 1.6 A) and a cooling system to dissipate the tremendous heat produced. Multiple gels may be electrotransferred in the standard field option, which is performed either at constant current (0.1 to 1 A) or voltage (5 to 30 V) from as little as 1 hour to overnight. The supported gel sandwich is placed vertically in a tank between stainless steel/platinum wire electrodes and the tank is filled with transfer buffer. The gel is then placed in the “transfer sandwich” (filter paper-gel-membrane-filter paper), cushioned by pads and pressed together by a support grid. When performing a wet transfer, the gel is first equilibrated in transfer buffer. The efficiency of protein transfer can be affected by the chemistry, thickness of the gel, the molecular weight of the proteins being transferred, the type of membrane and transfer buffers used, and the transfer method. In addition to the challenges of immunodetection in the protein blotting workflow, the transfer of proteins from a gel matrix to a membrane is a potential hurdle. An appropriate method is then used to detect the localized probe to document the location and relative abundance of the target protein. The transferred protein is then probed sequentially with antibodies and detection probe (e.g., enzyme, fluorophore, isotope). Better target protein accessibility on the membrane by macromolecules like antibodiesĪfter transfer, the membrane must be blocked to prevent non-specific binding of the antibody to the membrane surface.Better handling capability offered by the membrane compared to a fragile gel.Protein transfer from gel to membrane is necessary for two reasons: In procedures where protein separation is not required, the sample may be directly applied to the membrane by spotting using an approach called dot blotting. After electrophoresis, the separated proteins are transferred, or "blotted", onto a solid support matrix, usually a nitrocellulose or polyvinylidene difluoride (PVDF) membrane. The first step in a western blotting procedure is to separate the proteins in a sample by size using denaturing gel electrophoresis (i.e., sodium dodecyl sulfate polyacrylamide gel electrophoresis or SDS-PAGE) or native PAGE. That’s what makes this broccoli macaroni & cheese recipe so awesome. Top with panko and 1 tablespoon old bay seasoning. Combine melted butter and bread crumbs until bread crumbs have absorbed the butter. Pour over cooked, drained pasta and broccoli. Remove from heat and whisk in cheddar cheese, mustard, salt and pepper. Pour in the cooked mac and cheese and top with remaining cheeses. Reduce heat to low and simmer until thickened, about 2 minutes. (Will still look bit watery at this stage). Remove lid (Note 3), stir through cheese, then sprinkle over panko and parmesan. Remove from the oven, switch oven to broiler/grill on high. Place the lid on and bake for 20 minutes. ![]() Truth be told when I make this recipe I don’t always use macaroni noodles, I use penne, ronini or whatever is available! Maybe it isn’t actually mac & cheese at all?! Well it is delicious and a meal the whole family can enjoy! Try it this Broccoli Macaroni & Cheese out!Ĭooking for toddlers can be difficult, we do our best to make sure our kids eat meals we can all enjoy. Optional baking step: preheat oven to 375 degrees F and grease a baking dish with cooking spray. Add the macaroni and chicken, stir, then top with broccoli. Time to find a compromise! How about baked macaroni instead? Oh and lets sneak some broccoli in there for some veggie power while we are at it! We end up with broccoli cheesy goodness that the whole family can enjoy, and not feel completely guilty about! ![]() Add in nutritional yeast, mustard, tomato paste, garlic, salt and turmeric and cook until mixed though, about 1 minute. Having a toddler in the house we have plenty of requests to eat “mac & cheese” but sometimes the adults of the house are not quite as enthused. Heat coconut oil in a large saucepan over medium heat until warm. Broccoli Macaroni & Cheese! Baked macaroni is a more grown up way to enjoy the noodle and cheese classic. ![]() Īs production increased, the roster grew to include Canadian actress Emmanuelle Chriqui as Revetta Chess, Tammy Blanchard as Isabelle Allen, English actor Eamonn Walker as Howlin' Wolf, and comedian Cedric the Entertainer as Willie Dixon. According to director Martin, the role of James was written with Beyoncé in mind. Early announcements of the cast also included Columbus Short as Little Walter, Golden Globe winner Jeffrey Wright as Muddy Waters, and multi- Grammy Award winner Beyoncé as Etta James. Originally, Matt Dillon was slated to play the role of Chess, but the role was ultimately given to Adrien Brody due to scheduling conflicts with Dillon. Cadillac Records was produced by Andrew Lack and Sofia Sondervan, and co-executive produced by Beyoncé. Martin directed the film, financed by Sony BMG Film. Filming locations included Louisiana, Mississippi, and New Jersey. The filming of Cadillac Records started in February 2008. ![]() The screenplay was written by director Darnell Martin. Production Beyoncé at the premiere party for the release of the film The label started selling records from the back of Chess' Cadillac, and launched the careers of legendary musical personalities such as blues singers and harmonica and guitar players Little Walter and Muddy Waters, Howlin' Wolf, soul legend Etta James and guitarist singer-songwriters Chuck Berry and Willie Dixon. He ran the legendary company with his brother, Phil, through the 1950s and '60s. Leonard Chess was the co-founder of the 1950s American record label Chess Records, located in Chicago, Illinois. Elvis Presley as himself (archive footage uncredited).Vincent D'Onofrio as Mississippi DJ (uncredited).Eamonn Walker as Chester Burnett/Howlin' Wolf.Columbus Short as Walter Jacobs/Little Walter.Jeffrey Wright as McKinley Morganfield/Muddy Waters.Inevitably, business and personal lines blur as the sometimes-turbulent lives of the musicians play out. This attracts stars like Etta James ( Beyoncé), Howlin' Wolf ( Eamonn Walker) and Chuck Berry ( Mos Def). Waters' and Walter's success leads to Chess opening the doors for black musicians and beginning a new record label in 1950 – Chess Records. In 1947 in Chicago, a Jewish immigrant from Poland and bar owner Leonard Chess ( Adrien Brody) hires a blues combo, including guitarist Muddy Waters ( Jeffrey Wright) and harmonica player Little Walter ( Columbus Short). The soundtrack was released on Music World/Columbia and Sony Music. The film was released in North America on December 5, 2008, by TriStar Pictures. The film stars Adrien Brody as Leonard Chess, Cedric the Entertainer as Willie Dixon, Yasiin Bey as Chuck Berry, Columbus Short as Little Walter, Jeffrey Wright as Muddy Waters, Eamonn Walker as Howlin' Wolf, and Beyoncé as Etta James. The film explores the musical era from the early 1940s to the late 1960s, chronicling the life of the influential Chicago-based record-company executive Leonard Chess, and a few of the musicians who recorded for Chess Records. Cadillac Records is a 2008 American biographical drama film written and directed by Darnell Martin. ![]() As with other HACEK organisms, optimal growth of C hominis occurs in an environment containing supplemental carbon dioxide and high humidity. 14,15 C hominis is pleomorphic, appearing as pairs, chains, clusters, rosettes, or teardrops. In later years, however, it was further characterized and renamed C hominis. 5Ĭ hominis Bacillus: Described as having a Pasteurella-like appearance, C hominis was first called Group II D. 5,8,9 Growth of a small number of A actinomycetemcomitans colonies may be seen at 18 to 24 hours, but at least 48 hours of growth may be required. Like other HACEK organisms, A actinomycetemcomitans is an opportunistic pathogen that has been associated with meningitis, bacteremia, septic arthritis, osteomyelitis, central nervous system abscesses, thyroid abscesses, endophthalmitis, endocarditis, pericarditis, cellulitis, parotitis, UTIs, pneumonia, and periodontal infections. 9 A actinomycetemcomitans grows in broth and blood agar, often in granules that are attached to the side of the dish while the center of the dish remains clear. A actinomycetemcomitans may appear singularly, in pairs, or in clumps. 5,9 Most recently, it has been classified in the genus Aggregatibacter while maintaining the same species name. 5,9 Initially known as Bacterium actinomycetem comitans, it was reclassified as Haemophilus actinomycetemcomitans, but this classification is not preferred because of the organism’s lack of similarity to H influenzae. 13Ī Actinomycetemcomitans Bacillus: Aggregatibacter actinomycetemcomitans has been classified in various ways since its discovery. 13 H haemolyticus is often misidentified as Haemophilus influenzae, as some strains are not hemolytic, which is often the only characteristic used to differentiate the two types of bacteria. 9 H parainfluenzae is a pleomorphic coccobacillus that accounts for 75% of the flora of the upper respiratory tract. 9 H segnis, a small pleomorphic rod that often manifests in asymmetrical filamentous forms, is part of the normal flora of the mouth as well. 8,12Īphrophilus and paraphrophilus species, which are short rods, are part of the normal flora found in the mouth and pharynx of humans. Haemophilus species have been isolated from brain abscesses, meningitis, endocarditis, otitis media, sinusitis, epiglottitis, hepatic abscesses, intra-abdominal infections, pneumonia, necrotizing fasciitis, septic arthritis, osteomyelitis, postoperative surgical infections, and urinary tract infections (UTIs). 11 For the purposes of this review, all of the HACEK-causing species formally in the Haemophilus genus will be referred to by the genus name. H aphrophilus, H paraphrophilus, and H segnis recently were classified, along with A actinomycetemcomitans, into the genus Aggregatibacter. Haemophilus Species: Haemophilus species apart from the HACEK group include aphrophilus, haemolyticus, parahaemolyticus, parainfluenzae, paraphrophilus, and segnis. It is important to remember that there are other potential culture-negative species besides HACEK organisms, such as Legionella species, Bartonella species, Brucella species, and other fastidious pathogens. 1 With the advent of improved culture media and automated culture systems, prolonged incubation may no longer be necessary a 3- to 7-day incubation period may suffice. 8 Typically, HACEK organisms grow slowly and are subject to extended incubation periods of 7 to 21 days. 8,9 They grow well on nonspecific culture media rather than specialized agars like MacConkey’s agar, a growth medium for gram-negative bacteria. To grow, HACEK organisms need an atmosphere enriched with carbon dioxide. 4 This article will briefly review the HACEK pathogens, resulting infections, and common treatment approaches. 1,5-7 Rarely, HACEK organisms may cause severe systemic infections. 4 HACEK organisms are most often associated with infective endocarditis, accounting for up to 10% of cases. 2,3 These organisms commonly colonize the human oropharynx as normal, indigenous flora that could cause mouth infections. 1 The HACEK organisms include H aemophilus species, A ggregatibacter actinomycetemcomitans, C ardiobacterium hominis, E ikenella corrodens, and K ingella kingae. HACEK is an acronym for a group of organisms that are small, fastidious gram-negative bacilli. |
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